2018-06-29 17:26:202025-05-23 20:57:05
locus
BSU29190
BSU_29190
geneLength
957
960
outlinks
bsu
BSU29190
BSU_29190
Gene
Coordinates
2,986,588 → 2,987,547
2,986,588 2,987,547
Gene
Phenotypes of a mutant
essential according to [Pubmed|12682299], non-essential according to [Pubmed|23420519,20473954]
essential according to [Pubmed|12682299], non-essential according to [Pubmed|23420519,20473954]
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
ATP + β-D-fructose 6-phosphate --> ADP + β-D-fructose 1,6-bisphosphate + H+ (according to UniProt)
The protein
Protein family
phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
phosphofructokinase type A (PFKA) family (single member, according to UniProt)
The protein
[SW|Domains]
3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
3 x nucleotide binding domain (ATP) (2125), (154158), (171187)
The protein
Additional information
PfkA is a [SW|moonlighting protein]. [Pubmed|19193632]
extensive information on the structure and enzymatic properties of PfkA can be found at [http://www.proteopedia.org/wiki/index.php/Phosphofructokinase_(PFK) Proteopedia]
PfkA is a [SW|moonlighting proteins|moonlighting protein] [Pubmed|19193632]
extensive information on the structure and enzymatic properties of PfkA can be found at [http://www.proteopedia.org/wiki/index.php/Phosphofructokinase_(PFK) Proteopedia]
Biological materials
Mutant
GP590 (Δ[[gene|pfkA]]::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP595 (Δ[[gene|pfkA]]::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP1744: BSB1 Δ[[gene|pfkA]]::''aphA3'', available in [SW|Jörg Stülke]' lab
GP1747: Δ[[gene|pfkA]]::''aphA3'', available in [SW|Jörg Stülke]' lab, [Pubmed|27708634]
BKE29190 (Δ[[gene|pfkA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCATTCACCTCAGC, downstream forward: _UP4_TAATGTACAGCTGAAGGCTG
BKK29190 (Δ[[gene|pfkA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCATTCACCTCAGC, downstream forward: _UP4_TAATGTACAGCTGAAGGCTG
GP590 ([[gene|pfkA]]::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP595 ([[gene|pfkA]]::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP1744: BSB1 [[gene|pfkA]]::''aphA3'', available in [SW|Jörg Stülke]' lab
GP1747: [[gene|pfkA]]::''aphA3'', available in [SW|Jörg Stülke]' lab, [Pubmed|27708634]
BKE29190 ([[gene|pfkA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCATTCACCTCAGC, downstream forward: _UP4_TAATGTACAGCTGAAGGCTG
BKK29190 ([[gene|pfkA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCATTCACCTCAGC, downstream forward: _UP4_TAATGTACAGCTGAAGGCTG
Biological materials
Expression vector
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP87, available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], in [SW|pGP382]: pGP1266, available in [SW|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP393, available in [SW|Jörg Stülke]'s lab
for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1422, available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
The protein
Paralogous protein(s)
[[this]]
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP87, available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], in [SW|pGP382]: pGP1266, available in [SW|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP393, available in [SW|Jörg Stülke]'s lab
for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1422, available in [SW|Jörg Stülke]'s lab
labs
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]